《植物生理学报》 2015, 51(9): 1524-1528

植物亚显微结构中钾离子的原位检测技术

张莉, 王巧艳, 李思, 董鹤, 姚雅琴^*

西北农林科技大学生命科学学院, 陕西杨凌712100

通信作者：姚雅琴；E-mail: yaoyaqin@nwsuaf.edu.cn

摘　要：

利用亚硝酸钴铅钠[NaPbCo(NO₂)₆]与K⁺能形成KPbCo(NO₂)₆电子不透过性颗粒并沉淀在原位的原理, 以玉米叶片为材料, 建立了K⁺的原位检测方法。检测结果显示, K⁺主要分布在维管束、细胞膜、叶绿体、细胞核及核仁等部位, 与现有的结论相符。该方法显色特异性强、灵敏度高、处理的植物组织和细胞结构保持较完好, 因此, 可以作为一种新技术用于植物亚显微结构中K⁺的原位检测。

关键词：亚显微结构; K⁺; 原位检测

收稿：2015-05-05   修定：2015-08-31

资助：国家高技术研究发展计划项目(2011AA100504)。

In situ Detection Technology of Potassium in Submicroscopic Structures of Plants

ZHANG Li, WANG Qiao-Yan, LI Si, DONG He, YAO Ya-Qin^*

College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China

Corresponding author: YAO Ya-Qin; E-mail: yaoyaqin@nwsuaf.edu.cn

Abstract:

An in situ detection method for K⁺ was found with maize leaves as the materials in this paper. The distribution of sodium lead cobaltinitrite [NaPbCo(NO₂)₆] in submicroscopic structures of plant cells was observed by means of the production of KPbCo(NO₂)₆, which precipitated to form electronic impermeable particles in situ, when NaPbCo(NO₂)₆ reacted with K⁺. We found K⁺ is mainly distributed in the vascular bundles, cell membrane, chloroplasts, nuclei and nucleoli using this method, which is considerably consistent with current research results. The method has advantages of high sensitivity and specificity in coloration, with cell structure of plant tissue remaining relatively intact. Therefore, this method can be used as a new technology in detecting K⁺ in plant submicroscopic structures in situ.

Key words: submicroscopic structure; K⁺; in situ detection